Journal: bioRxiv

Article Title: Brood care in shell-dwelling cichlids is timed by independent maternal and larval clocks

doi: 10.1101/2023.12.22.573114

Figure Lengend Snippet: a) L. ocellatus male and female at the female’s shell buried in the sandy bottom of Lake Tanganyika (Photo credit Alex Jordan). b) L. ocellatus occupying and defending buried 3D printed shells in lab conditions. c) L. ocellatus father at his shell entrance with offspring inside the shell in our facility. d) Brood care stages of L. ocellatus : di) Mother laying eggs inside the first whorl of the shell nest, dii) hatched fry stay deep within the shell, diii) larvae are seen emerging from the shell, div) juveniles move across to the father’s shell. e) Construction of a 3D printable version of the Tanganyikan gastropod shell that allows camera footage access to the shell. f) Artificial extension of the dorso-ventral sides ensures access to deep chambers by adult L. ocellatus g) For analysis breakdown, the space inside the shell was divided into the entrance area (E; teal), laying chamber (L; blue) and deep chamber (D; navy). h) The tank set up includes a male and female breeding pair, each provided with a 3D printed shell attached to the glass and covered with a near infrared (NIR) -penetrable plastic to prevent visible light entering into the shell. i) The experimental timeline: Introduction of breeding pair occurs t days before fertilization. An NIR camera records images and videos from fertilization until the larvae are 11 days post-fertilisation (dpf) across daytime (L; white) and nighttime (D; gray).

Article Snippet: The time lapse ran from midnight at 7 dpf until 11:59 pm at 11 dpf with a NIR camera (Ximea, MX042RG-CM-X2G2-FF, Germany) with a 50 mm lens (Edmund Optics, Germany), filming from above.

Techniques:

Journal: bioRxiv

Article Title: Brood care in shell-dwelling cichlids is timed by independent maternal and larval clocks

doi: 10.1101/2023.12.22.573114

Figure Lengend Snippet: a) The experimental box used to test light preference with one side of the lid blocking visible light (black rectangle, NIR plastic) and the other allowing visible light through (white rectangle, clear). b) The mean percentage and standard error of larvae (n=28) that preferred the dark side of the box in each hour across 7-10 dpf. White and gray alternating bars under the plot indicate day and night, while vertical dotted gray lines separate each dpf c) A box plot of the percentage of larvae in the dark during the daytime before and after emergence. Each circle indicates the mean preference in each experiment before and after emergence. Overall difference is significant (asterisk) (p=0.03, Wilcoxon signed-rank test).

Article Snippet: The time lapse ran from midnight at 7 dpf until 11:59 pm at 11 dpf with a NIR camera (Ximea, MX042RG-CM-X2G2-FF, Germany) with a 50 mm lens (Edmund Optics, Germany), filming from above.

Techniques: Blocking Assay

Journal: Pharmaceutics

Article Title: Poisonous Caterpillar-Inspired Chitosan Nanofiber Enabling Dual Photothermal and Photodynamic Tumor Ablation

doi: 10.3390/pharmaceutics11060258

Figure Lengend Snippet: ( a ) Schematic illustration and hyperspectral images of anchored/detached AuDD from the CNf backbone at pH 7.4 and 6.8. ( b ) Changes in zeta potential of CNf-Ce6-based samples at pH 7.4 and 6.8 (mean ± SD, n = 3, ** p < 0.01 compared to pH 7.4). ( c ) Ce6 emission spectra (λ ex = 400 nm, λ em = 600–750 nm) for the free Ce6 or CNf-Ce6-based samples at pH 7.4 and 6.8. ( d ) 9,10-Dimethylanthracene fluorescence change (F f –F s ) in free Ce6 or CNf-Ce6-based samples at pH 7.4 and 6.8 (lower graphs), and NIR Ce6 fluorescence (λ ex = 635 nm, λ em = 720 nm) images of free Ce6 or CNf-Ce6-based samples at pH 7.4 and 6.8 (upper images). All samples were irradiated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source.

Article Snippet: Visualization of the CNf-based samples or AuDD at pH 7.4 or 6.8 was monitored using a Nikon microscope equipped with a visible and NIR hyperspectral camera (CytoViva, Auburn, AL, USA).

Techniques: Fluorescence, Irradiation

Journal: Pharmaceutics

Article Title: Poisonous Caterpillar-Inspired Chitosan Nanofiber Enabling Dual Photothermal and Photodynamic Tumor Ablation

doi: 10.3390/pharmaceutics11060258

Figure Lengend Snippet: ( a ) Uptake value [number (N) of internalized AuDD particles per MDA-MB-231 cell for 8 h of incubation at 37 °C] of the AuDD-based samples measured using an ICP-MS (mean ± SD, n = 3, ** p < 0.01 compared to AuDD at pH 7.4). ( b ) Flow cytometry analysis of MDA-MB-231 cells (incubation time: 8 h) treated with free Ce6, Ce6-tagged AuNP, BSA@CNf-Ce6, or AuDD/BSA@CNf-Ce6 at pH 7.4 and 6.8. ( c ) The hyperspectral images of the MDA-MB-231 cells incubated with untreated (control) or AuDD/BSA@CNf-Ce6 at pH 7.4 and 6.8.

Article Snippet: Visualization of the CNf-based samples or AuDD at pH 7.4 or 6.8 was monitored using a Nikon microscope equipped with a visible and NIR hyperspectral camera (CytoViva, Auburn, AL, USA).

Techniques: Incubation, Flow Cytometry